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KMID : 0364819880260020129
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Korean Journal of Microbiology
1988 Volume.26 No. 2 p.129 ~ p.136
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Effects of Some Carbohydrates and Ammonium Sulfate on Lignin Degradation by Pseudomonas diminuta
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김규중/Kim, Kyu-Jung
신광수/맨진수/강사욱/Kwang-Soo Shin/Maeng, Jin-Soo/Seong, Chi-Nam
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Abstract
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1
To investigate the influence of cosubstrate supplement and ammonium sulfate on lignin degradation by Pseudomonas diminuta KM4-2, isolated in the laboratory,the strain was cultured on the lignin media which contained ligpin as a source of carbon and the culture filtrate was analyzed by Sephadex G-75 column chromatography. It was found that polymerization was not appeared unlike wood-rot fungi. When the carbohydrates were added, the peak of lignin at 280nm by UV scanning spectra of the filtrate, was significantly increased. In order to determine the effect of ammonium sulfate on the ligninolytic activity, the isolated strain was incubated in the media containing 0.1%, 0.25% and 0.5% of nitrogen concentration in the Warburg flask and the rate of oxygen uptake was estimated by Warburg Respirometer. As a result, the activity was maximum at 0.1% of nitrogen concentration and thereafter decreased in parallel with nitrogen concentration.
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KEYWORD
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